Mascot Search Results

User            : 
Email           : 
Search title    : A few peptides from an LCMS run
MS data file    : U:\Mascot test data\TSQ\dyckall_1.asc
Database        : VarsplicC3  (198935 sequences; 59114902 residues)
Timestamp       : 2 Mar 2005 at 13:37:57 GMT
Significant hits: AAAADUF  AAAADUF
                  AAAADYD  AAAADYD
                  AAAADUC  AAAADUC
                  AAAADXG  AAAADXG
                  AAAADVY  AAAADVY

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 41 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (A few peptides from an LCMS run)

        Error tolerant   

1.     AAAADUF           Mass: 143338   Score: 183    Peptides matched: 3
  AAAADUF
Check to include this hit in error tolerant search or archive report
        
       Query   Observed   Mr(expt)   Mr(calc)    Delta  Miss  Score  Expect  Rank  Peptide
1   671.90   1341.78   1341.73   0.06  0   92   6.1e-007  1   DAGTISGLNVLR
2   808.30   1614.58   1613.76   0.83  0   71   6.7e-005  1   TTPSYVAFTDTER
4   1084.90   2167.78   2168.17   -0.39  1   26   1.1  1   IINEPTAAAIAYGLDKK

2.     AAAADYD           Mass: 110970   Score: 163    Peptides matched: 3
  AAAADYD
Check to include this hit in error tolerant search or archive report
        
       Query   Observed   Mr(expt)   Mr(calc)    Delta  Miss  Score  Expect  Rank  Peptide
  2   808.30   1614.58   1613.76   0.83  0   54   0.0031  2   TTPSYVAFTESER
3   973.90   1945.78   1945.92   -0.14  0   82   3.5e-006  1   NQVAMNPNNTIFDAK
  4   1084.90   2167.78   2168.17   -0.39  1   26   1.1  1   IINEPTAAAIAYGLDKK

3.     AAAADUC           Mass: 282114   Score: 133    Peptides matched: 3
  AAAADUC
Check to include this hit in error tolerant search or archive report
        
       Query   Observed   Mr(expt)   Mr(calc)    Delta  Miss  Score  Expect  Rank  Peptide
  2   808.30   1614.58   1613.76   0.83  0   54   0.0031  2   TTPSYVAFTESER
  3   973.90   1945.78   1945.92   -0.14  0   58   0.00085  2   NQVAMNPQNTVFDAK
  4   1084.90   2167.78   2168.17   -0.39  1   26   1.1  1   IINEPTAAAIAYGLDKK

4.     AAAADXG           Mass: 69912    Score: 129    Peptides matched: 2
  AAAADXG
Check to include this hit in error tolerant search or archive report
        
       Query   Observed   Mr(expt)   Mr(calc)    Delta  Miss  Score  Expect  Rank  Peptide
  2   808.30   1614.58   1613.76   0.83  0   71   6.7e-005  1   TTPSYVAFTDTER
  3   973.90   1945.78   1945.92   -0.14  0   58   0.00085  2   NQVAMNPQNTVFDAK

 
       Proteins matching the same set of peptides:
       AAAAEAN           Mass: 121799   Score: 129    Peptides matched: 2
  AAAAEAN
5.     AAAADVY           Mass: 84036    Score: 109    Peptides matched: 2
  AAAADVY
Check to include this hit in error tolerant search or archive report
        
       Query   Observed   Mr(expt)   Mr(calc)    Delta  Miss  Score  Expect  Rank  Peptide
  2   808.30   1614.58   1613.76   0.83  0   71   6.7e-005  1   TTPSYVAFTDTER
  3   973.90   1945.78   1946.94   -1.16  0   38   0.096  3   NQVAMNPTQTIFDAK

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : TrypsinJ
Fixed modifications    : HeavyJ,Oxidation (M),SMA (K),SMA (N-term)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 1 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : U:\Mascot test data\TSQ\dyckall_1.asc
Number of queries      : 4

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
dp210198c                      21-Jan-98 DERIVED SPECTRUM    #9
Score greater than 42 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit         Protein   Peptide
 92.1    0.06   1           AAAADUF  DAGTISGLNVLR
 49.3    1.05                        DAGTIAGLEVLR
 34.3    1.05                        LETLGINADIR
 33.4   -0.89                        VSGVGGSGGGRGGGR
 29.7   -0.91                        SVGTLGEADLK
 29.0    0.12                        NIFEQTPPNR
 28.5    1.05                        VSASATLPEALR
 27.8    1.04                        DARADTLAILR
 27.6    0.06                        DVETRVEVIR
 27.4    0.05                        GSSLIRAENIR
Top scoring peptide matches to query 2
dp210198b                      21-Jan-98 DERIVED SPECTRUM    #9
Score greater than 41 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit         Protein   Peptide
 70.6    0.83   1+          AAAADUF  TTPSYVAFTDTER
 54.0    0.83   2+          AAAADYD  TTPSYVAFTESER
 21.5   -0.23                        VCAIKAPGFGENR
 19.3   -0.14                        CYEVGMMKGGVR
 15.8    0.80                        TTPSYSGQYRTAR
 15.5   -0.15                        GSYTSESVEMARR
 14.9   -0.23                        IAMQGPGETSGIRR
 14.8   -0.32                        CVLPLDALHVPRR
 14.2    0.68                        TMAASALLAVVLGK
 13.7    0.90                        VAETDYEMETQR
Top scoring peptide matches to query 3
dp210198a                      21-Jan-98 DERIVED SPECTRUM    #9
Score greater than 40 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit         Protein   Peptide
 82.3   -0.14   2           AAAADYD  NQVAMNPNNTIFDAK
 58.5   -0.14   3+          AAAADUC  NQVAMNPQNTVFDAK
 37.9   -1.16   5           AAAADVY  NQVAMNPTQTIFDAK
 27.4   -0.20                        AMLPEVRPSSEVYAK
 22.4    0.86                        TDIETNMFPAAGSPPVR
 21.3    0.84                        NQLTTNPENTVFDAK
 19.5   -0.08                        DCSANTTSCHILGWGK
 17.8    1.79                        QLGIKNGMFFGHAK
 16.5    0.82                        MPPAFIKVENACTK
 16.1   -0.15                        FPARSDDVEVETELGR
Top scoring peptide matches to query 4
dp210198                       21-Jan-98 DERIVED SPECTRUM    #9
Score greater than 37 indicates homology
Score greater than 40 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit         Protein   Peptide
 26.4   -0.39   1+          AAAADUF  IINEPTAAAIAYGLDKK
 26.4   -0.39                        IINEPTAAALAYGIDKK
 26.4   -0.39                        IINEPTAAALAYGLDKK
 26.4   -0.39                        ILNEPTAAALAYGLDKK
 23.3   -0.39                        IINEPTAAALSFGLDKK
  8.9   -0.26                        TNCPNVSSFFQSNSVRVR
  8.9   -0.33                        IIHDNYNQLEVSSAALK
  8.8   -0.36                        IPQVVMTYEKLPEPK
  8.3   -1.47                        FTVPAAVLLTAIAYPILNR
  8.3    0.60                        LNIQSETQDIIGVTVRK