@article {49693,
	title = {Genetic depletion reveals an essential role for an SR protein splicing factor in vertebrate cells.},
	journal = {Bioessays},
	volume = {19},
	year = {1997},
	month = {1997 Mar},
	pages = {189-92},
	abstract = {<p>SR proteins are essential for the splicing of messenger RNA precursors in vitro, where they also alter splice site selection in a concentration-dependent manner. Although experiments involving overexpression or dominant mutations have confirmed that these proteins can influence RNA processing decisions in vivo, similar results with loss-of-function mutations have been lacking. Now, a system for genetic depletion of the chicken B cell line DT40 has revealed that the SR protein ASF/SF2 (alternative splicing factor/splicing factor 2) is essential for viability in these cells(1). This study opens the way for a complete functional dissection of this protein, and other SR proteins, in vivo.</p>},
	keywords = {Amino Acid Sequence, Animals, Molecular Sequence Data, Nuclear Proteins, RNA Splicing, RNA-Binding Proteins, Serine-Arginine Splicing Factors, Vertebrates},
	issn = {0265-9247},
	doi = {10.1002/bies.950190302},
	author = {Mount, S M}
}
