@article {49689,
	title = {Genomic sequence, splicing, and gene annotation.},
	journal = {Am J Hum Genet},
	volume = {67},
	year = {2000},
	month = {2000 Oct},
	pages = {788-92},
	keywords = {Animals, Consensus Sequence, Exons, genes, Genome, Genomics, HUMANS, Nucleotides, Regulatory Sequences, Nucleic Acid, RNA Splice Sites, RNA Splicing, Untranslated Regions},
	issn = {0002-9297},
	doi = {10.1086/303098},
	author = {Mount, S M}
}
@article {49701,
	title = {Species-specific signals for the splicing of a short Drosophila intron in vitro.},
	journal = {Mol Cell Biol},
	volume = {13},
	year = {1993},
	month = {1993 Feb},
	pages = {1104-18},
	abstract = {<p>The effects of branchpoint sequence, the pyrimidine stretch, and intron size on the splicing efficiency of the Drosophila white gene second intron were examined in nuclear extracts from Drosophila and human cells. This 74-nucleotide intron is typical of many Drosophila introns in that it lacks a significant pyrimidine stretch and is below the minimum size required for splicing in human nuclear extracts. Alteration of sequences of adjacent to the 3{\textquoteright} splice site to create a pyrimidine stretch was necessary for splicing in human, but not Drosophila, extracts. Increasing the size of this intron with insertions between the 5{\textquoteright} splice site and the branchpoint greatly reduced the efficiency of splicing of introns longer than 79 nucleotides in Drosophila extracts but had an opposite effect in human extracts, in which introns longer than 78 nucleotides were spliced with much greater efficiency. The white-apricot copia insertion is immediately adjacent to the branchpoint normally used in the splicing of this intron, and a copia long terminal repeat insertion prevents splicing in Drosophila, but not human, extracts. However, a consensus branchpoint does not restore the splicing of introns containing the copia long terminal repeat, and alteration of the wild-type branchpoint sequence alone does not eliminate splicing. These results demonstrate species specificity of splicing signals, particularly pyrimidine stretch and size requirements, and raise the possibility that variant mechanisms not found in mammals may operate in the splicing of small introns in Drosophila and possibly other species.</p>},
	keywords = {Animals, Base Sequence, Cell Nucleus, Consensus Sequence, DNA, DNA Transposable Elements, Drosophila, Drosophila Proteins, Electrophoresis, Polyacrylamide Gel, HeLa Cells, HUMANS, Introns, Molecular Sequence Data, Mutation, Peptide Hydrolases, Proteins, Regulatory Sequences, Nucleic Acid, Retroelements, RNA Splicing, Species Specificity},
	issn = {0270-7306},
	author = {Guo, M and Lo, P C and Mount, S M}
}
@article {49703,
	title = {Polyadenylylation in copia requires unusually distant upstream sequences.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {88},
	year = {1991},
	month = {1991 Apr 15},
	pages = {3038-42},
	abstract = {<p>Retroviruses and related genetic elements generate terminally redundant RNA products by differential polyadenylylation within a long terminal repeat. Expression of the white-apricot (wa) allele of Drosophila melanogaster, which carries an insertion of the 5.1-kilobase retrovirus-like transposable element copia in a small intron, is influenced by signals within copia. By using this indicator, we have isolated a 518-base-pair deletion, 312 base pairs upstream of the copia polyadenylylation site, that is phenotypically like much larger deletions and eliminates RNA species polyadenylylated in copia. This requirement of distant upstream sequences for copia polyadenylylation has implications for the expression of many genetic elements bearing long terminal repeats.</p>},
	keywords = {Animals, Base Sequence, Blotting, Northern, DNA Transposable Elements, Drosophila melanogaster, Eye Color, Molecular Sequence Data, Oligonucleotides, Polymerase Chain Reaction, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, RNA Processing, Post-Transcriptional, RNA, Messenger},
	issn = {0027-8424},
	author = {Kurkulos, M and Weinberg, J M and Pepling, M E and Mount, S M}
}
